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Antibacterial Effects of Grape Extracts on Helicobacter pylori.

Antibacterial Effects of Grape Extracts on Helicobacter pylori.










No.Divisions/Titles for AbstractDetails

1Abstract TitleAntibacterial Effects of Grape Extracts on Helicobacter pylori.

2Abstract SourceApplied and Environmental Microbiology, 75, 848-852.


3Abstract Author(s)Brown, J. C., Huang, G., Haley-Zitlin, V. & Jiang, X.


4Article AffiliationDepartment of Food Science & Human Nutrition, Clemson University, Clemson, South Carolina.


5AbstractAnti-Helicobacter pylori (substance active against the H-pylori )activities were determined by agar dilution,( method used by researchers to determine the resistance of pathogens to antibiotics) confocal laser scanning microscopy,(Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation) and cell proliferation (rapid increase in the number or amount of something.)assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phenolic levels do not necessarily determine overall anti-H. pylori efficacy.


6SummaryIn this study, muscadine grape skin extract was most effective against H. pylori in all tests performed. Although muscadine grape seed contained significantly higher total phenolic levels than other extracts, its efficacy against H. pylori was lower than or similar to that of muscadine skin and synergy extracts (Tables ​(Tables11 and ​and2),2), suggesting that higher phenolic levels do not necessarily correlate with increased anti-H. pylori activity but rather the type and concentrations of compounds present in these extracts. Resveratrol, the least-abundant major phenolic in muscadine grape skin, has been shown to inhibit cagA-positive H. pylori strains (6). Ellagic acid, the most-abundant phenolic, has also been reported to possess anti-H. pylori activity (15). In addition, this compound has been found to inhibit cancer, alter bacterial protein conformations, and affect ion pump function (11, 22); however, it is not known if a similar mode of action against H. pylori exists. Because these phenolics are partially hydrophobic, this may allow them to interact with the bacterial cell wall and lipopolysaccharide interfaces more effectively by decreasing membrane stability (25). Therefore, further research on individual compounds and their interactions in the fruit itself is necessary to elucidate the possible synergistic/antagonistic mechanism(s) by which these compounds exert their effects.Bacterial attachment to stomach epithelium is considered the initial step for H. pylori pathogenesis. Several recent studies have evaluated the antiadhesive properties of various natural compounds (1, 9, 17). Confocal microscopy revealed fewer bacterial cells present on host cell surfaces following treatment with grape extracts (Fig. ​(Fig.3),3), in agreement with AGS cell proliferation assays. Two possible explanations are that these compounds may interfere with H. pylori's ability to increase the expression of AGS cell adhesion molecules (10) or inhibit sialic acid-specific adhesions to epithelial cells (1).Following treatment with muscadine grape extracts or compounds, AGS cell proliferation was similar to that of the controls without H. pylori , suggesting that extracts either reduce or inhibit H. pylori from binding to and/or damaging AGS cells. 


7Article Published DateFeb 2009.

8Study TypeInvitro.

9SubstancesGrape Skin.

10DiseasesIBS.

11Pharmacological ActionsTreatment of IBS.

12Linkhttps://goo.gl/wz2fWP








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